Product Pathways - Metabolism
MTAP Antibody #4158
|4158S||100 µl (10 western blots)||---||In Stock||---|
|4158||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||29||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
MTAP Antibody detects endogenous levels of total MTAP protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the N terminus of human MTAP. Antibodies were purified by protein A and peptide affinity chromatography.
MTAP is an enzyme that is essential for the salvage pathway for both adenine and methionine synthesis. MTAP catalyzes the cleavage of 5’-methylthioadenosine into adenine and 5-methylthio-D-ribose-1-phosphate. Adenine is then used to generate AMP whereas 5-methylthio-D-ribose-1-phosphate is converted into methionine (1,2). MTAP is expressed in all normal cells and tissues, although frequently lost in different human tumors including pancreatic adenocarcinoma, neuroendocrine tumors, non-small cell lung carcinoma and breast carcinoma. MTAP is usually codeleted with p16 (cdkN2a/ARF) (3-5). MTAP overexpression in breast cancer cells inhibits their ability to form colonies in soft agar, thereby implicating its function as a tumor suppressor (6).
- Backlund, P.S. and Smith, R.A. (1981) J Biol Chem 256, 1533-5.
- Backlund, P.S. et al. (1982) J Biol Chem 257, 4196-202.
- Dreyling, M.H. et al. (1998) Genes Chromosomes Cancer 22, 72-8.
- Zhang, H. et al. (1996) Cancer Genet Cytogenet 86, 22-8.
- Illei, P.B. et al. (2003) Clin Cancer Res 9, 2108-13.
- Christopher, S.A. et al. (2002) Cancer Res 62, 6639-44.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.