Revision 1

#43191

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IF-F

Reactivity:
M

Sensitivity:
Endogenous

Source/Isotype:
Horse chimeric IgG4

UniProt ID:
#Q6QLQ4

Entrez-Gene Id:
56644

Product Usage Information

Application Dilution
Immunofluorescence (Frozen) 1:50 - 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Dectin-1/Clec7a (E3P5W) Horse Chimeric Monoclonal Antibody recognizes endogenous levels of total Dectin-1/Clec7a protein.

Source / Purification

This recombinant chimeric antibody is engineered from Dectin-1/Clec7a (E3P5W) Rabbit mAb #30260 according to animal-free protocols. The chimeric antibody retains its antigen-binding Fab regions from the original rabbit monoclonal antibody but contains a horse-derived Fc domain. When multiplexing, Fc-directed rabbit secondaries are required to detect rabbit-host primary antibodies.

The parent antibody, Dectin-1/Clec7a (E3P5W) Rabbit mAb #30260, is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro29 of mouse Dectin-1/Clec7a protein.

Description

This Cell Signaling Technology® antibody retains the antigen-binding Fab regions of the original parent host sequence from which it is engineered. This antibody is expected to exhibit the same species cross-reactivity as Dectin-1/Clec7a (E3P5W) Rabbit mAb #30260.

Background

Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Dectin-1/Clec7a (E3P5W) Horse Chimeric mAb (green), β-Amyloid (D54D2) XP® Rabbit mAb #8243 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). Rabbit primary antibody is detected using an anti-rabbit Fc-specific secondary antibody.
Immunofluorescence Image 1: Dectin-1/Clec7a (E3P5W) Horse Chimeric Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen brain from an amyloid mouse model of Alzheimer's disease using Dectin-1/Clec7a (E3P5W) Horse Chimeric mAb (gray), Iba1/AIF-1 (E4O4W) XP® Mouse Chimeric mAb #58410 (green), β-Amyloid (D54D2) XP® Rabbit mAb #8243 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue). Rabbit primary antibody is detected using an anti-rabbit Fc-specific secondary antibody.
Immunofluorescence Image 2: Dectin-1/Clec7a (E3P5W) Horse Chimeric Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.