Product Pathways - Lymphocyte Signaling
Phospho-AML1 (Ser249) Antibody #4327
|4327S||100 µl (10 western blots)||---||In Stock||---|
|4327||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Phospho-AML1 (Ser249) Antibody detects endogenous levels of AML1 protein only when phosphorylated on Ser249.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids around Ser249 of human AML1. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts of TPA treated HEL cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (upper), or AML1 Antibody #4334 (lower).
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with λ phosphatase (blue), using Phospho-AML1 (Ser249 ) Antibody compared to a nonspecific negative control antibody (red).
Confocal immunofluorescent analysis of HeLa cells, untreated or treated with λ phosphatase, using Phospho-AML1 (Ser249) Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
AML1 (also known as Runx1, CBFA2, and PEBP2αB) is a member of the core binding factor (CBF) family of transcription factors (1,2). It is required for normal development of all hematopoietic lineages (3-5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers: chromosomal translocations include t(8;21) AML1-ETO, t(12;21) TEL-AML, and t(8;21) AML-M2 (6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an Erk-dependent manner (7,8).
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- North, T.E. et al. (2004) Stem Cells 22, 158-168.
- Blyth, K. et al. (2005) Nat Rev Cancer 5, 376-387.
- Tanaka, T. et al. (1996) Mol Cell Biol 16, 3967-79.
- Zhang, Y. et al. (2004) J Biol Chem 279, 53116-25.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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