Revision 1

#44667

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
37

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9NYZ2

Entrez-Gene Id:
51312

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SLC25A37 (F6W1R) Rabbit Monoclonal Antibody recognizes endogenous levels of total SLC25A37 protein. This antibody detects bands of unknown origin at 60 and 70 kDa in some cell lines by western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp35 of human SLC25A37 protein.

Background

Mitochondria require iron import for heme and iron-sulfur cluster biogenesis for cellular respiration (reviewed in 1). Perturbation of iron homeostasis can manifest in several pathological conditions. Mitoferrin-1 (SLC25A37) and mitoferrin-2 (SLC25A28) are homologous members of the solute carrier family that are important for iron import into the mitochondria (2). Combinatorial CRISPR screening identified SLC25A37 and the mitochondrial glutathione transporter as important regulators supporting oxidative phosphorylation (3). Mitochondrial depolarization results in a PINK1/Parkin-dependent decrease in SLC25A37 and SLC25A28, and deletion of PINK and Parkin can accelerate K-Ras driven pancreatic tumorigenesis with an increase in mitochondrial iron accumulation (4). Deletion of SLC25A37 homologue in zebrafish inhibited erythroid maturation (5). Decreased expression of SLC25A37 has been described in erythropoietin protoporphyria, in which patients have an accumulation of protoporphyrins in red blood cells (6). Decreased expression of SLC25A37 has also been identified as a risk factor in major depressive disorder (7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using SLC25A37 (F6W1R) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of SLC25A37 protein in NCI-H146 and NCI-H69 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: SLC25A37 (F6W1R) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human SLC25A37 protein (hSLC25A37-Myc/DDK; +), using SLC25A37 (F6W1R) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: SLC25A37 (F6W1R) Rabbit Monoclonal Antibody
Immunoprecipitation of SLC25A37 protein from SU-DHL-1 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SLC25A37 (F6W1R) Rabbit mAb. Western blot analysis was performed using SLC25A37 (F6W1R) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: SLC25A37 (F6W1R) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.