Revision 2

#45003

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
SCA-ITS3

Reactivity:
H M

Sensitivity:
Endogenous

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q13541

Entrez-Gene Id:
1978

Storage

Supplied in PBS (pH 7.2), 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.

Specificity/Sensitivity

Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (InTraSeq™ 3' Conjugate 3021) detects endogenous levels of 4E-BP1 only when phosphorylated at Thr37 and/or Thr46. This antibody conjugate may cross-react with 4E-BP2 and 4E-BP3 when phosphorylated at equivalent sites. Non-specific staining has been observed in mitotic cells by immunofluorescence.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr37 and Thr46 of mouse 4E-BP1.

Background

Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

SCA-ITS3: Single Cell Analysis - InTraSeq™ 3'

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

10x Genomics, 10x, Feature Barcode, and Chromium are the trademarks or registered trademarks of 10x Genomics, Inc.

InTraSeq is a trademark of Cell Signaling Technology, Inc.

Subject to patents licensed from 10x Genomics, Inc. for use with single-cell (i.e., Chromium) 10x products.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Cultured human peripheral blood mononuclear cells (PBMCs) untreated (Cultured; left panel) or treated with Human IL-6 Recombinant Protein #48333, Human IL-2 Recombinant Protein #31058, Human IGF-I Recombinant Protein #29608, and Cell Stimulation Cocktail (without Protein Transport Inhibitors) (500X) #29255 (30 min, Stimulated; right panel) were processed in accordance with the InTraSeq™ 3’ protocol. The FeaturePlots in the figure display the Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb (InTraSeq™ 3' Conjugate 3021) expression on the Uniform Manifold Approximation and Projection (UMAP). To generate similar plots, use the FeaturePlot command template from the Seurat computational package: FeaturePlot(your_object, features = your_protein, max.cutoff = "q90", min.cutoff = "q10", order = TRUE) + scale_color_gradientn(colours = c("#22578B", "#73A9D2", "grey90", "#FDB393", "#b22222"))
SCA Image 1: Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit Monoclonal Antibody (InTraSeq<sup>™</sup> 3' Conjugate 3021)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.