Revision 2

#45181

Store at -20C

CD74 (D5N3I) & CO-0210-488 SignalStar® Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P04233

Entrez-Gene Id:
#972

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
CD74 (D5N3I) Rabbit Monoclonal Antibody (SignalStar® Conjugate 0210)7168250 µlHRabbit IgG
Complementary Oligo (CO-0210-488)6377830 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

CD74 (D5N3I) Rabbit Monoclonal Antibody (SignalStar® Conjugate 0210) recognizes endogenous levels of total CD74 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant human CD74 protein.

Background

CD74, which is also known as the MHC Class II-associated invariant chain (Ii), is a type II transmembrane glycoprotein that plays a critical role in the antigen presentation process as a chaperone of MHC Class II proteins. It is expressed at high levels on B cells and to a lesser extent on numerous antigen presenting cell (APC) types including dendritic cells, Langerhans cells, monocytes, and macrophages as well as non-traditional APCs such as epithelial cells (1,2). CD74 was initially identified for its ability to regulate the folding and intracellular trafficking of newly synthesized MHC Class II molecules. Following expression, CD74 self-assembles as a trimer that serves as a scaffold for the assembly of MHC Class II molecules. Through this interaction, CD74 blocks the peptide binding cleft of MHC Class II molecules and prevents their premature association with endogenous polypeptides (3). Binding to CD74 also facilitates the translocation of MHC Class II molecules from the endoplasmic reticulum to the endocytic compartments during antigen presentation (4). In addition to its role as an MHC Class II chaperone, CD74 is also the receptor for macrophage migration-inhibitory factor (MIF). Binding to CD74 and its co-receptor, CD44, has been shown to induce the activation of the NFkB and ERK pathways to promote cell proliferation and survival signals (5,6). Recent studies have identified CXCR2 and CXCR4 as co-receptors for CD74 where MIF binding to CD74 complexes contributes to MIF-mediated monocyte chemotaxis and the induction of Akt signaling, respectively (7,8). Increased CD74 surface expression has been reported under inflammatory conditions and in certain types of cancer cells implying a potential role in tumorigenesis (9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CD74 (D5N3I) Rabbit mAb (750; yellow), β2-microglobulin (D8P1H) Rabbit mAb (594; red), Ki-67 (8D5) Mouse mAb (647; white), Pan-Keratin (C11) Mouse mAb (647; cyan), CD3ε (D7A6E) Rabbit mAb (750; green), and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: CD74 (D5N3I) & CO-0210-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using CD74 (D5N3I) Rabbit mAb (594; yellow) and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: CD74 (D5N3I) & CO-0210-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded human anaplastic oligodendroglioma using CD74 (D5N3I) Rabbit mAb (left, 488; green) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human anaplastic oligodendroglioma using CD74 (D5N3I) Rabbit mAb #77274 (right). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: CD74 (D5N3I) & CO-0210-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded normal human gall bladder using CD74 (D5N3I) Rabbit mAb (647; red) and DAPI #4083 (blue).
Immunohistochemistry Image 1: CD74 (D5N3I) & CO-0210-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.