Product Pathways - NF-kB Signaling
RANK Ligand (L300) Antibody #4816
|4816S||100 µl (10 western blots)||---||In Stock||---|
|4816||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Monkey, Bovine, Pig.
Specificity / Sensitivity
RANK Ligand (L300) Antibody detects transfected levels of cellular RANK Ligand protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human RANK Ligand. Antibody was purified by protein A and peptide affinity chromatography.
RANK (receptor activator of NF-κB) is a member of the tumor necrosis factor (TNF) receptor subfamily that is activated by its ligand, RANKL (TRANCE/OPGL/ODF), to promote survival of dendritic cells and differentiation of osteoclasts (1-4). Although RANK is widely expressed, its cell surface expression may be more restricted to dendritic cells and foreskin fibroblasts (1). RANK contains a 383-amino acid intracellular domain that associates with specific members of the TRAF family to NF-κB and JNK activiation (1,5). RANKL/RANK signaling may also lead to survival signaling through activation of the Akt pathway and an upregulation of survival proteins, including Bcl-xL (2,6). RANK signaling has been implicated as a potential therapeutic to inhibit bone loss and arthritis (7,8).
RANKL (1), also named TNF-related activation-induced cytokine (TRANCE) (2,9), osteoprotegerin ligand (OPGL) (3), osteoclast differentiation factor (ODF) (4), and TNFSF11, is a type II transmembrane protein of the TNF family that exists as both a membrane-bound and soluble form. It is an essential regulator of immune function and bone development and homeostasis (7,10,11). RANKL is predominately expressed in activated T cells, as well as the thymus, lymph node, and bone marrow and promotes dendritic cell survival. Deletion of RANKL in mice leads to severe osteoporosis with a loss of osteoclasts, defects in T and B cell differentiation, loss of lymph node development, and mammary gland development during pregnancy (12-14).
- Anderson, D.M. et al. (1997) Nature 390, 175-9.
- Wong, B.R. et al. (1997) J. Exp. Med. 186, 2075-80.
- Lacey, D.L. et al. (1998) Cell 93, 165-76.
- Yasuda, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 3597-602.
- Darnay, B.G. et al. (1998) J. Biol. Chem. 273, 20551-5.
- Wong, B.R. et al. (1999) Mol. Cell 4, 1041-9.
- Walsh, M.C. and Choi, Y. Cytokine Growth Factor Rev. 14, 251-63.
- Nakashima, T. et al. (2003) Curr. Opin. Rheumatol. 15, 280-7.
- Wong, B.R. et al. (1997) J Biol Chem 272, 25190-4.
- Hofbauer, L.C. (1999) Eur J Endocrinol 141, 195-210.
- Theill, L.E. et al. (2002) Annu Rev Immunol 20, 795-823.
- Mizuno, A. et al. (1998) Biochem Biophys Res Commun 247, 610-5.
- Kong, Y.Y. et al. (1999) Nature 397, 315-23.
- Fata, J.E. et al. (2000) Cell 103, 41-50.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 3959 RANK Ligand (R2) Antibody
- 4845 RANK Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.