Revision 4

#49509

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IF-F, IF-IC, FC-FP

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
60-80

Source/Isotype:
Rabbit IgG

UniProt ID:
#P09086

Entrez-Gene Id:
5452

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence (Frozen) 1:200 - 1:800
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized) 1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #47603.

Specificity/Sensitivity

POU2F2 (F3B7M) Rabbit Monoclonal Antibody recognizes endogenous levels of total POU2F2 protein. This antibody is predicted to detect multiple human and mouse POU2F2 isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human POU2F2 protein.

Background

POU domain, class 2, transcription factor 2 (POU2F2), also known as Oct-2, is a transcription factor in the POU protein family (1-3). POU proteins contain two subunits: an N-terminal POU-specific domain and a C-terminal homeobox domain (1-3). Due to alternate splicing, POU2F2 has five human isoforms and seven mouse isoforms (3). POU2F2 is normally expressed in B cells and B cell lineage tumor cells, and is involved in B cell proliferation and differentiation by binding to immunoglobulin gene promoters (2-4). POU2F2 has also been shown to have a tumor-promoting role in some cancers, as well as potentially promoting oncogene-induced senescence (OIS) escape in early cancer progression (5,6).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using POU2F2 (F3B7M) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of POU2F2 protein in HeLa and C2C12 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with constructs expressing Myc/DDK-tagged full-length human POU2F2 protein (hPOU2F2-Myc/DDK; +) and Myc/DDK-tagged full-length mouse POU2F2 protein (mPOU2F2-Myc/DDK; +), using POU2F2 (F3B7M) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 2: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Immunoprecipitation of POU2F2 protein from Daudi cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is POU2F2 (F3B7M) Rabbit mAb. Western blot analysis was performed using POU2F2 (F3B7M) Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb (HRP Conjugate) #5127 was used as a secondary antibody.
Immunoprecipitation Image 1: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse lung using POU2F2 (F3B7M) Rabbit mAb (left, green) and co-labeled with CD45R/B220 (RA3-6B2) Rat mAb (redFluor 710 Conjugate) #82984 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue).
Immunofluorescence Image 1: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse spleen using POU2F2 (F3B7M) Rabbit mAb (green), CD3 (17A2) Rat mAb (FITC Conjugate) #86603 (blue pseudocolor), and CD45R/B220 (RA3-6B2) Rat mAb (redFluor 710 Conjugate) #82984 (red).
Immunofluorescence Image 2: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of Daudi cells (left, positive) and HeLa cells (right, negative) using POU2F2 (F3B7M) Rabbit mAb (green), Cytochrome c (6H2.B4) Mouse mAb #12963 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 4

Cell Signaling Technology Logo
Flow cytometric analysis of HDLM-2 cells (blue, negative) and Daudi cells (green, positive) using POU2F2 (F3B7M) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Flow cytometric analysis of C2C12 cells (blue, negative) and A20 cells (green, positive) using POU2F2 (F3B7M) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 2: POU2F2 (F3B7M) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.