Product Pathways - Adhesion
E-Cadherin (32A8) Mouse mAb #5296
|5296S||100 µl (10 western blots)||---||In Stock||---|
|5296||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
E-Cadherin (32A8) Mouse mAb detects endogenous levels of total E-cadherin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with human cancer cell lines. E-Cadherin (32A8) Mouse mAb recognizes an epitope in the extracellular region of E-cadherin.
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).
- Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35.
- Christofori, G. (2003) EMBO J 22, 2318-23.
- Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63.
- Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol 14, 427-34.
- Rabascio, C. et al. (2004) Cancer Res 64, 4373-7.
- Yamaoka-Tojo, M. et al. (2006) Arterioscler Thromb Vasc Biol 26, 1991-7.
- Patel, I.S. et al. (2003) Int J Cancer 106, 172-7.
- Sanders, D.S. et al. (2000) J Pathol 190, 526-30.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 3195 E-Cadherin (24E10) Rabbit mAb
- 3199 E-Cadherin (24E10) Rabbit mAb (Alexa Fluor® 488 Conjugate)
- 3396 TCF8/ZEB1 (D80D3) Rabbit mAb
- 9585 Slug (C19G7) Rabbit mAb
- 9589 Slug (L40C6) Mouse mAb
- 3879 Snail (C15D3) Rabbit mAb
- 3895 Snail (L70G2) Mouse mAb
- 4719 Snail (SN9H2) Rat mAb
- 9582 β-Catenin (6B3) Rabbit mAb
- 3390 Vimentin (5G3F10) Mouse mAb
- 3932 Vimentin (R28) Antibody
- 4061 N-Cadherin Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.