Revision 1

#53705

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-F, IF-IC

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
228

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q15154

Entrez-Gene Id:
5108

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Frozen) 1:400 - 1:800
Immunofluorescence (Immunocytochemistry) 1:100 - 1:400

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

PCM-1 (F8Q8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PCM-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PCM-1 protein.

Background

The centrosome is composed of a pair of centrioles surrounded by electron-dense pericentriolar material and functions as the microtubule-organizing center responsible for microtubule nucleation and spindle organization during cell cycle progression (1). Pericentriolar material 1 (PCM-1) is a large, 228 kDa protein associated with the centrosome in a cell cycle-dependent manner (2). PCM-1 localizes to small cytoplasmic granules called centriolar satellites (3). PCM-1 is required for the assembly of several centrosomal proteins, including centrin, pericentrin, ninein, NEK2, and CEP250 (4-8). Chromosomal translocations involving genes encoding PCM-1 and the tyrosine kinases Ret and Jak2 are associated with some cancers, including papillary thyroid carcinoma and myeloid leukemia (9-11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using PCM-1 (F8Q8B) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of PCM-1 protein in HCC1428 and HT-29 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: PCM-1 (F8Q8B) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with PCM-1 (F8Q8B) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (magenta), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: PCM-1 (F8Q8B) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse testis labeled with PCM-1 (F8Q8B) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: PCM-1 (F8Q8B) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse skeletal muscle labeled with PCM-1 (F8Q8B) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 3: PCM-1 (F8Q8B) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of A2780 cells (left, high-expressing) and HCC1428 cells (right, low-expressing) using PCM-1 (F8Q8B) Rabbit mAb (green) and DAPI #4083 (blue).
Immunofluorescence Image 1: PCM-1 (F8Q8B) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.