Product Pathways - DNA Damage
TERF2IP (D9H4) Rabbit mAb #5433
|5433S||100 µl (10 western blots)||---||In Stock||---|
|5433||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||55||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
TERF2IP (D9H4) Rabbit mAb detects endogenous levels of total TERF2IP protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human TERF2IP protein.
Telomeric repeat-binding factor 2-interacting protein (TERF2IP, also known as RAP1) is a component of the Shelterin Complex, a multi-protein complex that binds and organizes telomeres into T-loop structures to prevent them from being recognized by the cell as DNA double stranded breaks (1,2). The Shelterin Complex is composed of TERF2IP, TIN2 and TPP2 proteins, in addition to three DNA binding proteins that function to recruit the complex to telomeres: TRF1 and TRF2 bind double-stranded TTAGGG repeats found at telomeres, while the POT1 protein binds single-stranded TTAGGG repeats found at the very end of the telomeres (2). Together, these proteins function to protect telomeres and ensure proper replication and processing of chromosome ends. Recent studies have shown that TERF2IP is dispensable for maintenance of telomere length, organization of telomeric chromatin, and regulation of telomeric transcription (3,4). However, TERF2IP is required for inhibition of homology-directed repair (HDR), which can create undesirable telomeric sister chromatid exchange (3,4). In addition to its role in telomere maintenance, TERF2IP is also found in the cytoplasm, where it functions as an IκB kinase (IKK) adaptor protein and regulates NF-κB-dependent gene expression (5). TERF2IP forms a complex with IKKs and is critical for proper recruitment of IKKs to and activation of the p65 subunit of NF-κB. Elevated levels of TERF2IP have been found in breast cancer cells with NF-κB hyperactivity, and knockdown of TERF2IP sensitizes these cells to apoptosis, further identifying TERF2IP as a potential cancer therapeutic target (5).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 2645 Trf2 Antibody
- 3529 Trf1 (BED5) Mouse mAb
- 2682 IKKα Antibody
- 2684 IKKβ Antibody
- 2685 IKKγ Antibody
- 4812 IκBα (44D4) Rabbit mAb
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 4764 NF-κB p65 (C22B4) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.