Product Pathways - Cytoskeletal Signaling
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb #5617
|5617S||100 µl (10 western blots)||---||In Stock||---|
|5617||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Monkey||Endogenous||125||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Species predicted to react based on 100% sequence homology: Rat.
Specificity / Sensitivity
Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb detects endogenous levels of ARHGAP42 only when phosphorylated at Tyr376.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr376 of human ARHGAP42 protein.
Western blot analysis of extracts from COS-7 cells using Phospho-ARHGAP42 (Tyr376) (D45E9) Rabbit mAb. The phospho-specificity of the antibody was verified by preincubating the antibody with no peptide, phosphopeptide, or the corresponding non-phosphopeptide prior to incubating the membrane.
ARHGAP family members activate Rho GTPase proteins (1). Mutations of ARHGAP family genes lead to carcinogenesis through the dysregulation of Rho/Rac/Cdc42-like GTPases (2). Little is known about the biological function of ARHGAP42, a member of the ARHGAP family. ARHGAP42 contains a RhoGAP domain in the middle of its sequence, an SH3 binding domain at the C-terminal end of the protein sequence, as well as a BAR domain and a PH domain in the N-terminal region of the sequence, which indicate that the protein may function as GTPase activator and cytoskeletal adaptor.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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