Product Pathways - MAPK Signaling
|5633S||5 mg||---||In Stock||---|
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- Molecular Formula:
- Molecular Weight:
- 377.43 g/mol
Directions For Use
SB203580 is supplied as a lyophilized white powder. For 10 mM stock, resuspend 5 mg of the inhibitor in 1.32 ml DMSO. Aliquot and freeze at -20ºC to avoid multiple freeze/thaw cycles which can degrade the inhibitor. For experiments with cultured cells, we recommend pre-treating the cells with SB203580 at 10 µM for one to two hours prior to stimulation. This product is for in vitro research use only and is not intended for use in humans or animals.
Western blot analysis of extracts from NIH/3T3 cells, untreated or sorbitol-treated for 30 minutes with or without SB203580 pre-treatment (1 hr.), using Phospho-MAPKAPK-2 (Thr222) Antibody #3044 (upper) or MAPKAPK-2 Antibody #3042 (lower).
Western blot analysis of extracts from NIH/3T3 cells, untreated or sorbitol-treated for 30 minutes with or without SB203580 pre-treatment (1 hr.), using Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb #4511 (upper) or p38 MAPK Antibody #9212 (lower).
p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).
SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
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- 9913 Phospho-p38 MAPK Pathway Sampler Kit
- 9213 p38 MAPK Control Cell Extracts
- 4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb
- 9212 p38 MAPK Antibody
- 9210 PhosphoPlus® p38 MAPK (Thr180/Tyr182) Antibody Kit
- 3042 MAPKAPK-2 Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
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