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Store at -20C

Chaperone-Mediated Autophagy Antibody Sampler Kit

1 Kit

(6 x 20 microliters)

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Quantity Mol. Wt Isotype/Source
LAMP2A (F1A5A) Rabbit Monoclonal Antibody8119720 µl100-130 kDaRabbit IgG
LAMP2 (D5C2P) Rabbit Monoclonal Antibody4906720 µl100-130 kDaRabbit IgG
HSPA8 (D12F2) Rabbit Monoclonal Antibody844420 µl70-72 kDaRabbit IgG
HSP40 (C64B4) Rabbit Monoclonal Antibody487120 µl40 kDaRabbit
HSP90 (C45G5) Rabbit Monoclonal Antibody487720 µl90 kDaRabbit IgG
HOP (D10E2) Rabbit Monoclonal Antibody567020 µl64 kDaRabbit IgG
Anti-rabbit IgG, HRP-linked Antibody7074100 µlGoat

Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.

Description

The Chaperone-Mediated Autophagy Antibody Sampler Kit provides an economical means of detecting proteins involved in chaperone-mediated autophagy. The kit provides enough antibodies to perform two western blot experiments with each primary antibody.

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Background

Chaperone-mediated autophagy (CMA) is a process triggering the lysosomal-mediated degradation of specific protein targets (reviewed in 1,2). CMA is induced by a variety of stresses, including starvation, genotoxic insults, oxidative stress, and hypoxia. CMA upregulation has been linked to the survival and proliferation of cancer cells, while deficiencies in CMA have been described in many neurodegenerative pathologies. The mechanism of CMA is a multi-step process involving substrate recognition, trafficking to the lysosome, and degradation. Substrate recognition involves binding of target proteins containing a penta-peptide KFERQ, or KFERQ-like motif, to the cytosolic chaperone protein heat shock cognate protein 70 (HSC70), also called HSPA8 or HSP73. With the help of additional co-chaperones, including HSP90, HSP40, HOP, HIP, and BAG1, the HSC70-substrate complex is targeted to the lysosomal membrane where it interacts with lysosomal-associated membrane protein 2A (LAMP2A). LAMP2A is one of three splice variants of LAMP2 differing in a small carboxyl-terminal cytoplasmic tail. This region is required for lysosomal docking of HSC70–substrate complexes. Changes in LAMP2A expression through transcriptional regulation or protein stability factor in the regulation of CMA.

Background References

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.