Product Pathways - Protein Translation
Mili (D14F5) XP® Rabbit mAb #5940
|5940S||100 µl (10 western blots)||---||In Stock||---|
|5940P||40 µl (4 western blots)||---||In Stock||---|
|5940||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-F=Immunofluorescence (Frozen)
Specificity / Sensitivity
Mili (D14F5) XP® Rabbit mAb recognizes endogenous levels of total Mili protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the residues surrounding Leu118 of mouse Mili protein.
Western blot analysis of extracts from mouse testis and mouse brain using Mili (D14F5) XP® Rabbit mAb (upper) or β-Actin Antibody #4967 (lower).
Immunohistochemical analysis of paraffin-embedded mouse testis using Mili (D14F5) XP® Rabbit mAb.
Confocal immunofluorescent analysis of mouse testis using Mili (D14F5) XP® Rabbit mAb (green) and Pan-Keratin (C11) Mouse mAb #4545 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
The Drosophila piwi gene was identified as being required for the self-renewal of germ-line stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and human (1). Miwi and Mili proteins are both mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mouse (3-5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.