Product Pathways - Companion Products
Streptavidin (Magnetic Bead Conjugate) #5947
|5947S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|5947||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Streptavidin (Magnetic Bead Conjugate) is useful for the precipitation of biotinylated proteins (1,2). Recombinant streptavidin is immobilized by the covalent binding of primary amino groups with formylbenzamide-modified magnetic bead.
Directions For Use
Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.
For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.
Immunoprecipitation of phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) from NIH/ 3T3 cell extracts, treated with Human Platelet-Derived Growth Factor AA (hPDGF-AA) #8913 (10 min), using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E) XP® Rabbit mAb (Biotinylated) #4094 and Streptavidin (Magnetic Bead Conjugate) (lane 3). Lysate and Streptavidin (Magnetic Bead Conjugate) are shown in lane 2, demonstrating streptavidin bead binding. Lane 1 shows 10% lysate. Western blot analysis was performed using Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106.
Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Tween® is a registered trademark of ICI Americas, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.