Product Pathways - Cell Cycle / Checkpoint
SignalSilence® ATR siRNA II #6289
|6289S||300 µl (3 nmol)||---||In Stock||---|
|6289||carrier free and custom formulation / quantity||email request|
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Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® ATR siRNA I #6288 (+) or SignalSilence® ATR siRNA II (+), using ATR Antibody #2790 (upper) or α-Tubulin (11H10) Rabbit mAb #2125 (lower). The ATR Antibody confirms silencing of ATR expression, while the α-Tubulin (11H10) Rabbit mAb is used as a loading control.
SignalSilence® ATR siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ATR expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions For Use
CST recommends transfection with 100 nM ATR siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.
Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks (1-3). Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. While there have been no published reports of phosphorylation sites on ATR, Cell Signaling Technology has produced an antibody directed against phospho-ATR (Ser428) that demonstrates in vivo and UV-induced phosphorylation of this protein. This reagent could prove to be a valuable tool for monitoring ATR activation. Proline-directed phosphorylation sites like this one are often targeted by CDKs and MAPKs and can often dramatically affect protein conformation (4,5). Threonine 1989 was recently identified as a potential marker of activated ATR (6,7).
- Kastan, M.B. and Lim, D.S. (2000) Nat Rev Mol Cell Biol 1, 179-86.
- Abraham, R.T. (2004) DNA Repair (Amst) 3, 883-7.
- Shechter, D. et al. (2004) DNA Repair (Amst) 3, 901-8.
- Pinna, L.A. and Ruzzene, M. (1996) Biochim Biophys Acta 1314, 191-225.
- Zhou, X.Z. et al. (1999) Cell Mol Life Sci 56, 788-806.
- Nam, E.A. et al. (2011) J Biol Chem 286, 28707-14.
- Liu, S. et al. (2011) Mol Cell 43, 192-202.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
- 6568 SignalSilence® Control siRNA (Unconjugated)
- 6201 SignalSilence® Control siRNA (Fluorescein Conjugate)
- 6288 SignalSilence® ATR siRNA I
- 2790 ATR Antibody
- 2909 Phospho-(Ser/Thr) ATM/ATR Substrate (4F7) Rabbit mAb
- 2851 Phospho-(Ser/Thr) ATM/ATR Substrate Antibody
- 6966 Phospho-(Ser/Thr) ATM/ATR Substrate (S*/T*QG) (P-S/T2-100) Rabbit mAb
- 9607 Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
SignalSilence® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.