Revision 2

#63062

Store at -20C

Phospho-Tau (Thr205) Quantitative Control

10 µg

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

Web:

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
ELISA+

MW (kDa):
70

UniProt ID:
#P10636-8

Entrez-Gene Id:
4137

Description

Phospho-Tau (Thr205) Quantitative Control is a peptide based standard specific for FastScan™ Phospho-Tau (Thr205) ELISA Kit #51580 and Phospho-Tau (Thr205) Matched Antibody Pair #66673.

Directions for Use

Prior to use, thaw the quantitative control and briefly spin down to collect all liquid at the bottom of the tube. Dilute according to the following instructions depending on the ELISA kit type. An example sensitivity curve is shown in Figure 2.

PathScan® ELISA Kits:
Dilute with ELISA Sample Diluent to achieve a 1X protein concentration.

PathScan® RP ELISA Kits:
Dilute with 1X Cell Lysis Buffer #9803 to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the detection antibody.

FastScan™ ELISA Kits:
Dilute with 1X FastScan™ ELISA Cell Extraction Buffer to a 2X protein concentration to achieve a final 1X protein concentration upon addition of the antibody cocktail.

Formulation

Supplied in 1X PBS (8.1 mM Na2HPO4, 2.68 mM KCl, 1.675 mM KH2PO4, and 137 mM NaCl (pH 7.4)), 0.4% BSA, and 0.25% ProClin.

Storage

Store at -20ºC.

Purity

Assessed to be > 93% by densitometry using SDS-PAGE analysis and Coomassie Blue detection.

Background

Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by Erk, glycogen synthase kinase-3 (GSK-3), and CDK5 (1,2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease (AD); these tangles are bundles of paired helical filaments (PHFs) composed of hyperphosphorylated tau. In particular, phosphorylation at Ser396 by GSK-3 or CDK5 destabilizes microtubules. Furthermore, research studies have shown that inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1,3).

Background References

Applications Key

ELISA+: ELISA and/or ELISA-like Assays

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

#63062

Phospho-Tau (Thr205) Quantitative Control

Cell Signaling Technology Logo
Figure 1. The purity of Phospho-Tau (Thr205) Quantitative Control is shown by SDS-PAGE staining with Coomassie Blue. Phospho-Tau (Thr205) Quantitative Control has a predicted molecular weight of 70 kDa.
ELISA and/or ELISA-like Assays Image 1: Phospho-Tau (Thr205) Quantitative Control
Figure 2. Titration curve demonstrating the relationship between concentration of Phospho-Tau (Thr205) Quantitative Control #63032 and the absorbance at 450 nm using the FastScan™ Phospho-Tau (Thr205) ELISA Kit #51580.
ELISA and/or ELISA-like Assays Image 2: Phospho-Tau (Thr205) Quantitative Control
Orders: 877-616-CELL (2355) • [email protected] • Support: 877-678-TECH (8324) • [email protected] • Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.