Revision 1

#64996

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-IC

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
160

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q15424

Entrez-Gene Id:
6294

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunofluorescence (Immunocytochemistry) 1:400 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SAFB1 (F5Z6A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SAFB1 protein. This antibody does not cross-react with SAFB2 protein. This antibody detects a 75 kDa band of unknown origin in mouse and rat. Reactivity by immunofluorescence is human only.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala909 of human SAFB1 protein.

Background

Scaffold attachment factor B1 and B2 (SAFB1/B2) are homologs that bind to scaffold or matrix attachment region DNA elements (S/MAR DNA) (1,2). SAFB1 and SAFB2 bind to estrogen receptor to inhibit its activity, an action enhanced by tamoxifen treatment and opposed by histone deacetylase activity (2-4). SAFB1 and SAFB2 contain an RNA recognition motif and can interact with various hnRNP complexes and serine/arginine-rich splicing factors to influence pre-mRNA splicing (5-7). SAFB1 is also capable of helping in the DNA damage response by allowing yH2AX spreading (8). Knockdown of SAFB1 results in increased SAFB2 expression, and knockdown of SAFB2 results in increased SAFB1 expression (9). Despite their high homology, SAFB1 and SAFB2 have some non-redundant functions. SAFB1 knockout mice have a high degree of growth and fertility issues, while SAFB2 knockout mice have a normal phenotype but aberrations in Sertoli cells (10). SAFB proteins are expressed lowly in breast cancers with poor prognosis (11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using SAFB1 (F5Z6A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: SAFB1 (F5Z6A) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-), human SAFB1 siRNA (hSAFB1 siRNA; +), or human SAFB2 siRNA (hSAFB2 siRNA; +), using SAFB1 (F5Z6A) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb (lower).
Western Blotting Image 2: SAFB1 (F5Z6A) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of HCT 116 cells, mock transfected (left, high-expressing) or transfected with siRNA directed against SAFB1 (right, low-expressing), using SAFB1 (F5Z6A) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: SAFB1 (F5Z6A) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.