Revision 3

#65540

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IHC-P

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
90

Source/Isotype:
Rabbit IgG

UniProt ID:
#P78563

Entrez-Gene Id:
104

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunohistochemistry (Paraffin) 1:800 - 1:3200

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ADAR2 (F6U8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total ADAR2 protein. This antibody detects a non-specific band of unknown origin at 180 kDa that might represent the ADAR2 homodimer.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ADAR2 protein.

Background

ADAR2, also known as RED1, is an enzyme that catalyzes the adenosine (A) to inosine (I) editing of double-stranded RNA (dsRNA) (1). This is the most common type of RNA editing in mammals, and because inosine is read as guanosine by the cellular machinery, ADAR2 can effectively alter genetic information at the RNA level (2,3). ADAR2 is known to be efficient and perform site-specific editing, thereby modulating encoded protein properties (4). ADAR2 can influence RNA stability by modifying the access of RNA-binding proteins, particularly decay-promoting factors (5), and has also been shown to affect alternative splicing (6). Importantly, ADAR2 forms a homodimer complex, which is critical for its functions (7,8). Dysregulation of ADAR2 activity has been implicated in various human diseases, including amyotrophic lateral sclerosis (ALS), where reduced ADAR2-mediated editing of GluA2, a subunit of the L-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor, has been observed (9). ADAR2 has also been linked to cancer, with altered ADAR2 expression correlating with malignancy in different tumor types. ADAR2 also edits numerous miRNAs and contributes to tumorigenesis regulation by balancing the functions of oncogenic and tumor-suppressive miRNAs (10,11). ADAR2 can also display tumor-suppressive functions. For example, ADAR2 is downregulated in approximately 50% of hepatocellular carcinoma (HCC), and overexpression of ADAR2 in ADAR-deficient HCC cells reduces their oncogenic potential (12).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using ADAR2 (F6U8S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Low expression of ADAR2 protein in U-87 MG and TT cells is consistent with the predicted expression pattern.
Western Blotting Image 1: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Western blot analysis of extracts from Neuro-2a, C6, and COS-7 cells using ADAR2 (F6U8S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Simple WesternTM analysis of lysates (0.1 mg/mL) from VCAP cells using ADAR2 (F6U8S) Rabbit mAb #65540.  The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody.  The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody.  This experiment was performed under reducing conditions on the JessTM Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western Blotting Image 1: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the parotid gland using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 1: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 2: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human ovarian serous papillary carcinoma using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 3: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 4: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 5: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Panel-

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma (left), ovarian carcinoma (middle) or normal brain (right) using ADAR2 (F6U8S) Rabbit mAb (top) or an ADAR Rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on human ADAR. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.
Immunohistochemistry Image 6: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 7: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using ADAR2 (F6U8S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 8: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded SET-2 cell pellet (left, high-expressing) or U-87 MG cell pellet (right, low-expressing) using ADAR2 (F6U8S) Rabbit mAb.
Immunohistochemistry Image 9: ADAR2 (F6U8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.