Revision 2

#66083

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IHC-P, IF-F, IF-IC, FC-FP, ELISA

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
50

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q8TBX8

Entrez-Gene Id:
79837

Product Usage Information

This product is the carrier free version of product #61407. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #61407

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total PIP4K2C protein. This antibody does not cross-react with PIP4K2A or PIP3K2B proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu321 of human PIP4K2C protein.

Background

Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).

PIP4K2C, also known as phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with the highest levels in kidney and brain tissue (9). PIP4K2C can form a homodimer or heterodimer with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling, and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry) FC-FP: Flow Cytometry (Fixed/Permeabilized) ELISA: ELISA

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Western blot analysis of extracts from various human, mouse, and rat cell lines using PIP4K2C (E7P3S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of PIP4K2C protein in TALL-1 and REH cells is consistent with the predicted expression pattern. Data were generated using the standard formulation of this product.
Western Blotting Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected (+) with constructs expressing HA-tagged full-length human PIP4K2A (hPIP4K2A-HA), HA-tagged full-length human PIP4K2B (hPIP4K2B-HA), or Myc/DDK-tagged full-length human PIP4K2C (hPIP4K2C-Myc/DDK), using PIP4K2C (E7P3S) Rabbit mAb (upper), HA-Tag (C29F4) Rabbit mAb #3724 (upper-middle), Myc-Tag (71D10) Rabbit mAb #2278 (lower-middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human papillary thyroid carcinoma using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 2: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 3: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human kidney using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 4: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human testis using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 5: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded normal human stomach using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 6: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 7: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse brain using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 8: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded mouse testis using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 9: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using PIP4K2C (E7P3S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).  Data were generated using the standard formulation of this product.
Immunohistochemistry Image 10: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded COLO 201 cell pellet (left, positive) or REH cell pellet (right, negative) using PIP4K2C (E7P3S) Rabbit mAb. Data were generated using the standard formulation of this product.
Immunohistochemistry Image 11: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum labeled with PIP4K2C (E7P3S) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with AQP4 (D1F8E) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #89851 (red), GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (magenta), and DAPI #4083 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Confocal immunofluorescent analysis of Colo 201 cells (left, positive) or REH cells (right, negative) using PIP4K2C (E7P3S) Rabbit mAb (green) and DAPI #4083 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 2

Cell Signaling Technology Logo
Flow cytometric analysis of TALL-1 cells (blue, negative) and REC-1 cells (green, positive) using PIP4K2C (E7P3S) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.
Flow Cytometry Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Data using PIP4K2C Matched Antibody Pair #53637 are shown. The two antibodies function as a capture-target-detection sandwich (#84254 and #66083, respectively) to detect endogenous protein expression in positive and negative controls.
ELISA Image 1: PIP4K2C (E7P3S) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.