Revision 1

#66951

Store at -20C

Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P05023

Entrez-Gene Id:
#476

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
Na,K-ATPase α1 (D4Y7E) Rabbit mAb (SignalStar™ Conjugate 0079)2616350 µlHRabbit IgG
Complementary Oligo (CO-0079-488)1878122 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

Na,K-ATPase α1 (D4Y7E) Rabbit mAb (SignalStar™ Conjugate 0079) recognizes endogenous levels of total Na,K-ATPase α1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human Na,K-ATPase α1 protein.

Background

The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients. It is composed of a catalytic α subunit and a β subunit (reviewed in 1). Several phosphorylation sites have been identified for the α1 subunit. Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5). All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase. Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6). Na,K-ATPase is also involved in other signal transduction pathways. Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the α subunit (7). Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using PCNA (D3H8P) & CO-0090-594 SignalStar™ Oligo-Antibody Pair #49873 (green), Na,K-ATPase α1 (D4Y7E) & CO-0079-647 SignalStar™ Oligo-Antibody Pair #16129 (magenta), and OX40L (D6K7R) & CO-0087-750 SignalStar™ Oligo-Antibody Pair #95054 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Na,K-ATPase α1 (D4Y7E) & CO-0079-488 SignalStar™ Oligo-Antibody Pair #66951 (green). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human small cell lung carcinoma using Na,K-ATPase α1 (D4Y7E) & CO-0079-594 SignalStar™ Oligo-Antibody Pair #85191 (yellow). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastrointestinal stromal tumor using Na,K-ATPase α1 (D4Y7E) & CO-0079-647 SignalStar™ Oligo-Antibody Pair #16129 (red). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 4: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human colorectal adenocarcinoma using Na,K-ATPase α1 (D4Y7E) & CO-0079-750 SignalStar™ Oligo-Antibody Pair #31205 (cyan). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 5: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma using Na,K-ATPase α1 (D4Y7E) & CO-0079-594 SignalStar™ Oligo-Antibody Pair #85191 (left, yellow) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human gastric adenocarcinoma using Na,K-ATPase α1 (D4Y7E) Rabbit mAb #23565 (right). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 6: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded colorectal adenocarcinoma using OX40L (D6K7R) & CO-0087-488 SignalStar™ Oligo-Antibody Pair #49516 (green), CD14 (D7A2T) & CO-0085-594 SignalStar™ Oligo-Antibody Pair #74041 (yellow), PCNA (D3H8P) & CO-0090-647 SignalStar™ Oligo-Antibody Pair #60626 (cyan), MHC Class I (EMR8-5) & CO-0082-750 SignalStar™ Oligo-Antibody Pair #55370 (magenta), CD141/Thrombomodulin (E7Y9P) & CO-0088-488 SignalStar™ Oligo-Antibody Pair #82890 (red), and CD8α (D4W2Z) & CO-0040-647 SignalStar™ Oligo-Antibody Pair #57623 (white). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 7: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human tonsil using PCNA (D3H8P) & CO-0090-488 SignalStar™ Oligo-Antibody Pair #36181 (magenta), Na,K-ATPase α1 (D4Y7E) & CO-0079-594 SignalStar™ Oligo-Antibody Pair #85191 (cyan), and OX40L (D6K7R) & CO-0087-647 SignalStar™ Oligo-Antibody Pair #73292 (red). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 1: Na,K-ATPase alpha1 (D4Y7E) & CO-0079-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.