Revision 10

#71810

Store at -20C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, W-S, IP, IHC-P

Reactivity:
H M R

Sensitivity:
Endogenous

MW (kDa):
55

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q96SW2

Entrez-Gene Id:
51185

Product Usage Information

Application Dilution
Western Blotting 1:1000
Simple Western™ 1:10 - 1:50
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #46351.

Specificity/Sensitivity

CRBN (D8H3S) Rabbit Monoclonal Antibody recognizes endogenous levels of total CRBN protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro44 of human CRBN protein.

Background

CRBN/Cereblon, a substrate receptor for the CRL4 E3 ubiquitin ligase, was originally identified for its role in autosomal recessive nonsyndromic mental retardation (1). CRBN’s role in the regulation of the AMPK pathway has been linked to various human diseases, including cardiovascular disease and obesity, and its involvement in proteasomal degradation may be important in neuropsychiatric diseases (2). CRBN has also been shown to enhance the effects of immunomodulatory imide drugs (IMiDs) in multiple myeloma, and may be a predictive marker for therapy (2-4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting W-S: Simple Western™ IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human M: Mouse R: Rat

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CRBN (D8H3S) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 1: CRBN (D8H3S) Rabbit Monoclonal Antibody
Simple Western™ analysis of lysates (1 mg/mL) from 3T3 cells using CRBN (D8H3S) Rabbit mAb #71810. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Western Blotting Image 1: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunoprecipitation of CRBN protein from TF-1 extracts. Lane 1 is CRBN (D8H3S) Rabbit mAb #71810, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control#3900, and lane 3 is 10% input. Western blot analysis was performed using CRBN (D8H3S) Rabbit mAb #71810. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 was used as the secondary antibody.
Immunoprecipitation Image 1: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 1: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using CRBN (D8H3S) Rabbit mAb
Immunohistochemistry Image 2: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human adenoid cystic carcinoma of the salivary gland using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 3: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using CRBN D8H3S) Rabbit mAb.
Immunohistochemistry Image 4: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 5: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human colon using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 6: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human spleen using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 7: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human testis using CRBN D8H3S) Rabbit mAb.
Immunohistochemistry Image 8: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 9: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 10: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using CRBN (D8H3S) Rabbit mAb.
Immunohistochemistry Image 11: CRBN (D8H3S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human normal testis (left), prostate adenocarcinoma (middle), and colon carcinoma (right) using CRBN (D8H3S) Rabbit mAb (top) or a CRBN rabbit mAb (bottom). These two antibodies detect unique, non-overlapping epitopes on CRBN protein. The similar staining patterns obtained with both antibodies to help to confirm the specificity of the staining.
Immunohistochemistry Image 12: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 10

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using CRBN (D8H3S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 13: CRBN (D8H3S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.