Revision 3

#72247

Store at +4C

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
IHC-P

Reactivity:
H

Sensitivity:
Endogenous

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q01101

Entrez-Gene Id:
3642

Product Usage Information

Application Dilution
Immunohistochemistry (Paraffin) 1:800 - 1:3200

Storage

Supplied in a PBS-based buffer with ~1% BSA and less than 0.1% sodium azide. Stable for 6 months when stored at 4°C. Do not aliquot the antibody.

Note: This product is only available in the format listed above. We are unable to provide this in a carrier free, custom formulation.

Specificity/Sensitivity

INSM1 (BLR272L) Rabbit Monoclonal Antibody recognizes endogenous levels of INSM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a peptide representing a region between residue 50 and 100 of human INSM1 protein.

Background

Insulinoma-associated protein 1 (INSM1), originally named IA-1, is a zinc-finger protein cloned from an insulinoma and glucagonoma subtraction library (1). It is a transcriptional repressor expressed in the developing pancreas and nervous system and targets genes such as NeuroD/β2 and insulin, critical regulators of neuroendocrine differentiation during development (2-5). Most importantly, INSM1 has emerged as a key marker in neuroendocrine tumor types, including small cell lung cancer (SCLC), medullary thyroid carcinoma, pheochromocytoma, Merkel cell carcinoma, neuroblastoma, and neuroendocrine tumors of the pancreas, breast, colon, bladder, and prostate (6-9). Some studies have also shown that the level of INSM1 expression may have prognostic value (10,11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

IHC-P: Immunohistochemistry (Paraffin)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human neuroendocrine carcinoma of the gallbladder using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 1: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine tumor using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 2: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 3: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded human non-small cell lung carcinoma using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 4: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human pancreas using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 5: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human small intestine using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 6: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded normal human lymph node using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 7: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded normal human colon using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 8: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded human duodenal neuroendocrine tumor metastasized to the lymph node using INSM1 (BLR272L) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 9: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded SCLC-21H cell pellet (left, positive) or A-431 cell pellet (right, negative) using INSM1 (BLR272L) Rabbit mAb.
Immunohistochemistry Image 10: INSM1 (BLR272L) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.