Revision 1

#72742

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, FC-FP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
80-100

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q86UZ6

Entrez-Gene Id:
140685

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50
Flow Cytometry (Fixed/Permeabilized) 1:500 - 1:1500

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ZBTB46 (F6Z3F) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZBTB46 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ZBTB46 protein.

Background

Zinc finger and BTB domain-containing protein 46 (ZBTB46) is a member of the broad complex zinc finger protein family, which plays a critical role in transcriptional regulation of immune response, tumor microenvironment, and hematopoietic differentiation (1-3). ZBTB46 is often expressed in dendritic cells (DCs) and their committed progenitors, and is used as a marker to distinguish them from other immune cell types (3,4). ZBTB46 has been shown to act as a transcriptional repressor in these cell types, maintaining a homeostatic steady state; however, it was shown to be non-essential for DC development (3,8). ZBTB46 is also expressed in quiescent endothelial cells and was shown to inhibit cell proliferation (3,4). Recent work has focused on the role of ZBTB46 in tumors. In breast cancer, ZBTB46 was shown to upregulate a long non-coding RNA (lncRNA) that can control breast cancer progression by manipulating tumor immune response (5). A fusion gene consisting of ZBTB46 and WFDC13 is a novel therapeutic target candidate in high-grade serous ovarian carcinoma (6). ZBTB46 is highly expressed and promotes upregulation of inflammation response and neuroendocrine differentiation genes in prostate cancer (7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using ZBTB46 (F6Z3F) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). Negative expression of ZBTB46 protein in Ramos and HT-1376 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: ZBTB46 (F6Z3F) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ZBTB46 protein (hZBTB46-Myc/DDK; +), using ZBTB46 (F6Z3F) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: ZBTB46 (F6Z3F) Rabbit Monoclonal Antibody
Immunoprecipitation of ZBTB46 protein from RD cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ZBTB46 (F6Z3F) Rabbit mAb. Western blot analysis was performed using ZBTB46 (F6Z3F) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: ZBTB46 (F6Z3F) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Flow cytometric analysis of fixed/permeabilized Ramos cells (blue, negative) and SU-DHL-1 cells (green, positive) using ZBTB46 (F6Z3F) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow Cytometry Image 1: ZBTB46 (F6Z3F) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.