Revision 1

#76291

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H M R Mk

Sensitivity:
Endogenous

MW (kDa):
270

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q01082

Entrez-Gene Id:
6711

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:100

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody recognizes endogenous levels of total SPTBN1 protein. This antibody detects a 50 kDa and a 70 kDa protein of unknown identity in some cell lines.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala2176 of human SPTBN1 protein.

Background

β-II spectrin (SPTBN1), the most common isoform of non-erythrocyte spectrin, is a cytoskeleton protein in many cell types (1). SPTBN1 is a large actin cross-linking and scaffold protein that links the cell membrane and cytoskeleton. It is crucial for not only cell shape and organization but also a variety of cellular functions, such as cell adhesion, spreading, cell cycle regulation, and apoptosis (1,2).SPTBN1 is involved in cell growth and development (particularly in brain) via the TGF-β/Smad signaling pathway (3,4). SPTBN1 knockout mice show defects in cortical organization, developmental delay, and behavioral deficiencies (3), and variants in human SPTBN1 have been strongly implicated in intellectual disabilities and autism (4). Aberrant expression of SPTBN1 in various cell and tissue types has been shown in cancer development and progression, particularly ovarian, breast, and liver cancers (5-8). SPTBN1 plays an anticancer role in many tumor types. It assists in chemotherapeutic resistance and decreases the migration ability of tumor cells (5,6), highlighting its role in metastatic tumor progression and its potential as a pharmaceutical target (7-9). Recent work has shown that SPTBN1 is important for controlling CAR-T cell migration targeting tumors (10).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human M: Mouse R: Rat Mk: Monkey

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using SPTBN1 (F8Y1H) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower). This antibody detects a 50 kDa and a 70 kDa protein of unknown identity in some cell lines. Negative expression of SPTBN1 protein in LNCaP and BV-2 cells is consistent with the predicted expression pattern.
Western Blotting Image 1: SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody
Immunoprecipitation of SPTBN1 protein from C2C12 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is SPTBN1 (F8Y1H) Rabbit mAb. Western blot analysis was performed using SPTBN1 (F8Y1H) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: SPTBN1 (F8Y1H) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.