Revision 1

#76304

Store at -20C

TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P09758

Entrez-Gene Id:
#4070

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
TACSTD2/TROP2 (F4W4J) Rabbit mAb (SignalStar™ Conjugate 0150)7503550 µlHRabbit IgG
Complementary Oligo (CO-0150-488)2542322 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

TACSTD2/TROP2 (F4W4J) Rabbit mAb (SignalStar™ Conjugate 0150) recognizes endogenous levels of total TACSTD2/TROP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TACSTD2/TROP2 protein. The antigen has been further characterized as corresponding to residues surrounding Ile219 of human TACSTD2/TROP2 protein.

Background

TROP2 is a transmembrane glycoprotein encoded by gene TACSTD2 (tumor-associated calcium signal transducer 2). TROP2 was first discovered as a biomarker of invasive trophoblast cells and later reported in many types of cancer cells, in various organs during development, and adult stem cells during homeostasis (1,2). TROP2 has an extracellular domain with EGF thyroglobulin type-1 repeats, a transmembrane domain, and a short cytoplasmic tail with a HIKE domain containing a PIP2 binding site and PKC phosphorylation site (Ser303) (1-4). TROP2 functions by regulating multiple signaling pathways, including the interaction of its extracellular domain with integrin beta1 to regulate FAK signaling, the association of its transmembrane domain with claudin-1 and claudin-7 for tight junction formation, and the regulation of intracellular calcium release by its PIP2 binding and activation of the ERK/MAPK pathway (1,2,5-8). All these functions are important for its role in tumor proliferation, metastasis, and invasion (1,2). PKC can phosphorylate TROP2 at Ser303; the phosphorylation changes the cytoplasmic tail conformation and further promotes its signaling (9). TROP2 can be activated through intramembrane proteolysis first by TACE, followed by further cleavage by Presenilin 1 and Presenilin 2. The proteolysis process is required for its role in tumor cell proliferation (10,11).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using EpCAM (D9S3P) & CO-0145-594 SignalStar™ Oligo-Antibody Pair #52009 (yellow), VE-Cadherin (E6N7A) & CO-0149-647 SignalStar™ Oligo-Antibody Pair #95571 (red), TACSTD2/TROP2 (F4W4J) & CO-0150-750 SignalStar™ Oligo-Antibody Pair #64488 (cyan), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems. 
Immunohistochemistry Image 1: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using TACSTD2/TROP2 (F4W4J) & CO-0150-750 SignalStar™ Oligo-Antibody Pair #64488 (cyan) and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. Staining was performed on the BOND RX autostainer by Leica Biosystems. 
Immunohistochemistry Image 2: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell lung carcinoma using TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar™ Oligo-Antibody Pair #76304 (green) and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 1: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using TACSTD2/TROP2 (F4W4J) & CO-0150-594 SignalStar™ Oligo-Antibody Pair #90680 (yellow) and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 2: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using TACSTD2/TROP2 (F4W4J) & CO-0150-647 SignalStar™ Oligo-Antibody Pair #94656 (red) and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 3: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
SignalStar™ multiplex immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using VE-Cadherin (E6N7A) & CO-0149-488 SignalStar™ Oligo-Antibody Pair #53594 (green), TACSTD2/TROP2 (F4W4J) & CO-0150-594 SignalStar™ Oligo-Antibody Pair #90680 (yellow), and DAPI #4083 (blue). All fluorophores have been assigned a pseudocolor, as indicated. 
Immunohistochemistry Image 4: TACSTD2/TROP2 (F4W4J) & CO-0150-488 SignalStar<sup>™</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.