Revision 1

#79842

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
78

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9C0B5

Entrez-Gene Id:
25921

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody recognizes endogenous levels of total ZDHHC5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu330 of human ZDHHC5 protein.

Background

Zinc finger DHHC domain-containing protein 5 (ZDHHC5) is a multipass membrane protein that is localized predominantly at the plasma membrane and endosomal compartment membranes (1). ZDHHC5 belongs to a large family of enzymes known as palmitoyl-acyltransferases. Using long-chain fatty acids such as palmitic acid, ZDHHC5 catalyzes the reversible, post-translational modification of cysteine residues within protein substrates to regulate their trafficking and subcellular localization (1,2). Research studies have shown that ZDHHC5 is itself regulated by multiple types of post-translational modifications, such as phosphorylation and palmitoylation, which regulate its subcellular localization and enzymatic activity (3,4). Palmitoylation mediated by ZDHHC5 plays a role in the host response to pathogens. Indeed, ZDHHC5-mediated palmitoylation of NOD1/2 pattern recognition receptors facilitates their correct localization to endosomal vesicle membranes containing internalized bacteria in order to initiate innate immune signaling cascades (5). Furthermore, ZDHHC5 was recently shown to interact with SARS-CoV-2 spike protein. This interaction is posited to facilitate S-acylation of the spike protein and fusion with host cell membranes (6,7).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various human cell lines using ZDHHC5 (E7T4N) Rabbit mAb.
Western Blotting Image 1: ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, transfected with control siRNA (-) or ZDHHC5 siRNA (+), using ZDHHC5 (E7T4N) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ZDHHC5 (hZDHHC5-Myc/DDK; +), using ZDHHC5 (E7T4N) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 3: ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Immunoprecipitation of ZDHHC5 protein from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ZDHHC5 (E7T4N) Rabbit mAb. Western blot analysis was performed using ZDHHC5 (E7T4N) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: ZDHHC5 (E7T4N) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.