Product Pathways - Neuroscience
GGA3 (D66F1) Rabbit mAb #8027
|8027S||100 µl (10 western blots)||---||In Stock||---|
|8027||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||90||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
GGA3 (D66F1) Rabbit mAb recognizes endogenous levels of total GGA3 protein. This antibody may also recognize cross-reacting bands of unknown origin between 25 and 32 kDa.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human GGA3 protein.
Western blot analysis of extracts from rat brain, NIH/3T3 cells, and mouse brain using GGA3 (D66F1) Rabbit mAb.
GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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