Revision 1

#81150

Store at -20C

Glut1 (E4S6I) & CO-0192-594 SignalStar® Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P11166

Entrez-Gene Id:
#6513

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
Glut1 (E4S6I) Rabbit Monoclonal Antibody (SignalStar™ Conjugate 0192)7124450 µlH MRabbit IgG
Complementary Oligo (CO-0192-594)4675730 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

Glut1 (E4S6I) Rabbit Monoclonal Antibody (SignalStar™ Conjugate 0192) recognizes endogenous levels of total Glut1 protein. This antibody does not cross-react with Glut2, Glut3, or Glut4.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Glut1 protein.

Background

Glucose transporter 1 (Glut1, SLC2A1) is a widely expressed transport protein that displays a broad range of substrate specificity in transporting a number of different aldose sugars as well as an oxidized form of vitamin C into cells (1,2). Glut1 is responsible for the basal-level uptake of glucose from the blood through facilitated diffusion (2). Research studies show that Glut1 and the transcription factor HIF-1α mediate the regulation of glycolysis by O-GlcNAcylation in cancer cells (3). Additional studies demonstrate that Glut1 is required for CD4 T cell activation and is critical for the expansion and survival of T effector (Teff) cells (4). Mutations in the corresponding SLC2A1 gene cause GLUT1 deficiency syndromes (GLUT1DS1, GLUT1DS2), a pair of neurologic disorders characterized by delayed development, seizures, spasticity, paroxysmal exercise-induced dyskinesia, and acquired microcephaly (5,6). Two other neurologic disorders - dystonia-9 (DYT9) and susceptibility to idiopathic generalized epilepsy 12 (EIG12) - are also caused by mutations in the SLC2A1 gene (7,8).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using CD38 (E9F5A) Rabbit mAb (594; magenta), CD86 (E5W6H) Rabbit mAb (488; white), Glut1 (E4S6I) Rabbit mAb (594; red), CD8α (D4W2Z) Rabbit mAb (647; green), F4/80 (D2S9R) Rabbit mAb (750; yellow), and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: Glut1 (E4S6I) & CO-0192-594 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded mouse liver using Glut1 (E4S6I) Rabbit mAb (750; cyan) and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: Glut1 (E4S6I) & CO-0192-594 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Glut1 (E4S6I) Rabbit mAb (left, 594; yellow) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded human prostate adenocarcinoma using Glut1 (E4S6I) Rabbit mAb #73015 (right). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: Glut1 (E4S6I) & CO-0192-594 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Glut1 (E4S6I) Rabbit mAb (488; green), CD20 (E7B7T) Rabbit mAb (594; yellow), CD44 (E7K2Y) Rabbit mAb (647; red), MMP-9 (D6O3H) Rabbit mAb (750; cyan), and DAPI #4083 (blue).
Immunohistochemistry Image 1: Glut1 (E4S6I) & CO-0192-594 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded normal human colon adjacent to colon carcinoma using Glut1 (E4S6I) Rabbit mAb (647; red), MMP-9 (D6O3H) Rabbit mAb (750; cyan), and DAPI #4083 (blue).
Immunohistochemistry Image 2: Glut1 (E4S6I) & CO-0192-594 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.