Product Pathways - Growth Factors/Cytokines
Human Interleukin-7 (hIL-7) #8170
|8170LF||50 µg (Carrier Free)||---||In Stock||---|
|8170LC||50 µg (With Carrier)||---||In Stock||---|
|8170SC||10 µg (With Carrier)||---||In Stock||---|
|8170SF||10 µg (Carrier Free)||---||In Stock||---|
Already purchased this product? Write a Review.
Recombinant human IL-7 (hIL-7) Asp26-His177 (Accession #NP_000871) was produced in E. coli at Cell Signaling Technology.
Recombinant hIL-7 has a Met on the amino terminus and has a calculated MW of 17,518. DTT-reduced and non-reduced protein migrate as 17 kDa polypeptides. The expected amino-terminal MDCDI of recombinant hIL-7 was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-7. All lots are greater than 98% pure.
The bioactivity of recombinant hIL-7 was determined in a 2E8 cell proliferation assay. The ED50 of each lot is between 90-1000 pg/ml.
The purity of recombinant hIL-7 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-7 and staining overnight with Coomassie Blue.
Less than 0.01 ng endotoxin/1μg hIL-7.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-7. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
IL-7 plays a key role in lymphopoiesis and lymphoid homeostasis (1). Stromal and epithelial cells within the bone marrow and thymus produce IL-7 (1). The primary targets of IL-7 are T cells, B cells, and dendritic cells (1). IL-7 is crucial for T cell development, the importance of which is underscored by the lack of T cells in both mice and humans that are deficient in IL-7/IL-7R signaling (1,2). While IL-7 appears to be required for B cell development in mice, the role of IL-7 in human B cell development is unclear (1,3). In addition to its effects on T cell lymphopoiesis, IL-7 promotes the maintenance and survival of naïve and memory αβ T cells, as well as γδ T cells (1). The IL-7 receptor is a heterodimer of the common γ chain, γc, and the IL-7-specific IL-7Rα (1). IL-7 activates PI3K/Akt, Jak1/2, and Stat1, 3, and 5 (1).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.