Revision 3

#84039

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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IF-IC, ChIP, ChIP-seq

Reactivity:
H

Sensitivity:
Endogenous

MW (kDa):
42

Source/Isotype:
Rabbit IgG

UniProt ID:
#P78545

Entrez-Gene Id:
1999

Product Usage Information

For optimal ChIP and ChIP-seq results, use 10 μL of antibody and 10 μg of chromatin (approximately 4 × 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunofluorescence (Immunocytochemistry) 1:800 - 1:3200
Chromatin IP 1:50
Chromatin IP-seq 1:50

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

ELF3 (F8J2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELF3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ELF3 protein.

Background

The E26 transformation-specific (ETS) transcription factors bind to a core 5’-GGAA/T-3’ DNA motif and are known to regulate many biological processes, including cell proliferation, differentiation, apoptosis, hematopoiesis, and angiogenesis; they have also been described in pathological conditions, including cancer (reviewed in 1). ETS transcription factor E74-like factor 3 (ELF3), also called ESE-1, ESX, Jen, and ERT, belongs to a subtype of ETS proteins characterized by a predominant epithelial-specific expression profile (2-6). ELF3 shows widespread expression across tissues with high expression in the digestive tract. Targeted knockout of the ELF3 gene in mice results in lethality in about 30% of the embryos to E11.5, along with abnormalities in the intestinal epithelium (7). ELF3 can have dualistic roles in cancer with tumor-promoting and suppressing activity (reviewed in 8,9). Dysregulation of ELF3 through genetic and epigenetic mechanisms such as inactivating mutations, promoter methylation, and gene amplification have been reported across multiple cancer types and stages of disease. In addition, ELF3 has been shown to play a role in immune disorders and inflammation (10-12).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IF-IC: Immunofluorescence (Immunocytochemistry) ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq

Cross-Reactivity Key

H: Human

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using ELF3 (F8J2G) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Negative expression of ELF3 protein in NCI-H1299 and Jurkat cells is consistent with the predicted expression pattern.
Western Blotting Image 1: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Immunoprecipitation of ELF3 protein from HT55 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is ELF3 (F8J2G) Rabbit mAb. Western blot analysis was performed using ELF3 (F8J2G) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunoprecipitation Image 1: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of HCC-78 cells (left, positive) and NCI-H1299 cells (right, negative) using ELF3 (F8J2G) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue).
Immunofluorescence Image 1: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 3

Cell Signaling Technology Logo
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCC-78 cells and ELF3 (F8J2G) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figure shows binding across GABPB2 gene.
Chromatin Immunoprecipitation Image 1: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCC-78 cells and ELF3 (F8J2G) Rabbit mAb, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. DNA Library was prepared using DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795. The figures show binding across chromosome 1 (upper), including GABPB2 gene (lower).
Chromatin Immunoprecipitation Image 2: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Chromatin immunoprecipitations were performed with cross-linked chromatin from HCC-78 cells and either ELF3 (F8J2G) Rabbit mAb or Normal Rabbit IgG #2729, using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005. The enriched DNA was quantified by real-time PCR, using human GABPB2 promoter primers, human SELL promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Chromatin Immunoprecipitation Image 3: ELF3 (F8J2G) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.