Product Pathways - Apoptosis
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate) #8788
|8788S||100 µl (50 tests)||---||In Stock||---|
|8788||carrier free and custom formulation / quantity||email request|
Already purchased this product? Write a Review.
Applications Key: F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Bovine, Pig.
Specificity / Sensitivity
Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb #9579.
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Pacific Blue™ is a trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.