Product Pathways - Cytoskeletal Signaling
Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #8856
|8856S||100 µl (50 tests)||---||In Stock||---|
|8856||carrier free and custom formulation / quantity||email request|
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|IF-IC||1:50||Human, Mouse, Rat||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Monkey, Dog.
* Product-specific protocol.
Specificity / Sensitivity
Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of keratin 17 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17 protein.
Flow cytometric analysis of A549 (blue) and HeLa (green) cells using Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).
Confocal immunofluorescent analysis of HeLa (keratin 17 positive; left), HCC827 (keratin 17 negative, keratin 19 positive; middle), or SK-MEL-28 (keratin 17/19 negative; right) cells using Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087 (red).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Keratin 17 (D73C7) XP® Rabbit mAb #4543.
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
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For Research Use Only. Not For Use In Diagnostic Procedures.
XP® is a trademark of Cell Signaling Technology, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.