Product Pathways - Neuroscience
Phospho-CREB (Ser133) Antibody #9191
|9191L||300 µl (30 western blots)||---||In Stock||---|
|9191S||100 µl (10 western blots)||---||In Stock||---|
|9191||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat||Endogenous||43||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-CREB (Ser133) Antibody detects endogenous levels of CREB only when phosphorylated at serine 133. This antibody also detects the phosphorylated form of the CREB-related protein ATF-1, and has been shown to react weakly with overexpressed levels of pyruvate dehydrogenase.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human CREB. Antibodies are purified by protein A and peptide affinity chromatography.
CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
- Lonze, B.E. et al. (2002) Neuron 34, 371-85.
- Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
- Redmond, L. et al. (2002) Neuron 34, 999-1010.
- Dash, P.K. et al. (1990) Nature 345, 718-21.
- Yin, J.C. et al. (1994) Cell 79, 49-58.
- Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
- Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
- Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
- Tan, Y. et al. (1996) EMBO J 15, 4629-42.
- Grewal, S.S. et al. (2000) J Biol Chem 275, 34433-41. Applications: ICC-IF.
- Ho, N. et al. (2000) J Neurosci 20, 6459-72. Applications: IHC-F (floating).
- Huang, H. et al. (2001) J Biol Chem 276, 38830-6. Applications: Western Blotting.
- Hu, B.R. et al. (1999) Neuroscience 89, 437-52. Applications: ICC-IF, IHC-F (floating).
- Pláteník, J. et al. (2005) J Neurochem 95, 1446-60. Applications: Western Blotting.
- Sakaguchi, M. et al. (2008) Mol Biol Cell 19, 78-85. Applications: Western Blotting.
- Almeida, L.E. et al. (2009) J Neurosci 29, 12702-10. Applications: Western Blotting.
- Gehani, S.S. et al. (2010) Mol Cell 39, 886-900. Applications: Western Blotting.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
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