Product Pathways - MAPK Signaling
PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit #9230
|9230S||1 Kit (10 western blots)||---||In Stock||---|
|9230||carrier free and custom formulation / quantity||email request|
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|Kit Includes||Quantity||Applications||Reactivity||Mol. Wt.||Isotype|
|Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody #9231||100 µl||W||H, M, R||40 Phospho-MKK3. 41 Phospho-MKK6. kDa||Rabbit|
|MKK3 Antibody #5674||100 µl||W||H, M, R, Mk||40 kDa||Rabbit|
|Anti-rabbit IgG, HRP-linked Antibody #7074||100 µl||Goat|
|Anti-biotin, HRP-linked Antibody #7075||100 µl||Goat|
|20X LumiGLO® Reagent and 20X Peroxide #7003||5 ml each||W|
|Biotinylated Protein Ladder Detection Pack #7727||100 µl|
|MKK3/MKK6 Control Cell Extracts #9233||200 µl|
Applications Key: W=Western Blotting
Reactivity Key: H=Human, M=Mouse, R=Rat, Mk=Monkey
Western blot analysis of extracts from various cell lines using MKK3 Antibody #5674.
The PhosphoPlus® MKK3/MKK6 (Ser189/207) Antibody Kit provides reagents and protocols for the rapid analysis of the phosphorylation status of MKK3/MKK6 at Ser189/207.
Specificity / Sensitivity
Phospho-MKK3/MKK6 (Ser189/207) Antibody detects endogenous levels of MKK3/MKK6 only when phosphorylated at Ser189/207. The antibody does not recognize the corresponding phosphorylated residues of MEK1, MEK2 or MKK4/SEK1. MKK3 Antibody detects endogenous levels of total MKK3 protein. This antibody does not cross-react with MEK1, MEK2, MKK4 or MKK6.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser189/207 of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human MKK3. Antibodies are purified by protein A and peptide affinity chromatography.
MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
PhosphoPlus® is a trademark of Cell Signaling Technology, Inc.
LumiGLO® is a registered trademark of Kirkegaard & Perry Laboratories.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.