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Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W

Reactivity:
H M

Sensitivity:
Endogenous

MW (kDa):
250

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q9Y2I7

Entrez-Gene Id:
200576

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

PIKFYVE (E4X3R) Rabbit Monoclonal Antibody recognizes endogenous levels of total PIKFYVE protein.

Species predicted to react based on 100% sequence homology

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr1653 of human PIKFYVE protein.

Background

PIKFYVE is a lipid kinase that phosphorylates position 5 of PIP (phosphatidylinositol) or PI(3)P (phosphatidylinositol 3-phosphate) to form PI5P or PI(3,5)P2 (1,2). The normal cellular levels of the latter two lipids are important in maintaining the functional responses of endosomes, lysosomes, and autophagosomes, as well as the regulation of cell cycle, stress response, T cell activation, and chromatin remodeling (3,4). PIKFYVE functions within a regulatory complex including the PIKFYVE kinase, a scaffold protein Vac14, and a lipid phosphatase Fig4 (Sac) (5,6). The complex localizes to endosomes and lysosomes, regulating endosome-to-TGN retrograde transport, lysosome fission, and lysosomal related autophagy (7-10). Targeted disruption of the PIKFYVE gene is embryonic lethal, indicating the vital role of the kinase in early embryonic development (11). Inhibition of PIKFYVE disrupts lysosome function in autophagy and can selectively kill certain cancer cells (12,13). PIKFYVE inhibition also potentiates immune response to checkpoint blockade in the tumor microenvironment (14).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting

Cross-Reactivity Key

H: Human M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.