Product Pathways - Jak/Stat Pathway
Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb #9314
|9314S||100 µl (10 western blots)||---||In Stock||---|
|9314||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||90||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Rat, Monkey, Bovine.
* Product-specific protocol.
Specificity / Sensitivity
Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr694 of Stat5.
Western blot analysis of extracts from K562, untreated or treated with Gleevec for 1 hour, using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb (upper) or Stat5 Antibody #9310 (lower).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.
Immunohistochemical analysis using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb on SignalSlide® Phospho-Stat1/3/5 IHC Controls #8105 (paraffin-embedded HeLa cell pellets, untreated (left), treated with Human Interferon-α1 (hIFN-α1) #8927 (middle), or treated with Human Epidermal Growth Factor (hEGF) #8916 (right).
Immunohistochemical analysis of paraffin-embedded K562 cell pellets control (left) or STI-571-treated (right) using Phospho-Stat5 (Tyr694) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.
Flow cytometric analysis of K562 cells, Gleevec-treated (blue) or untreated (green), using Phospho-Stat5 (Tyr694) (C71E5) Rabbit mAb.
Stat5 is activated in response to a wide variety of ligands including IL-2, GM-CSF, growth hormone and prolactin. Phosphorylation at Tyr694 is obligatory for Stat5 activation (1,2). This phosphorylation is mediated by Src upon erythropoietin stimulation (3). Stat5 is constitutively active in some leukemic cell types (4). Phosphorylated Stat5 is found in some endothelial cells treated with IL-3, which suggests its involvement in angiogenesis and cell motility (5). Stat5a and Stat5b are independently regulated and activated in various cell types. For instance, interferon treatment predominantly activates Stat5a in U-937 cells and Stat5b in HeLa cells (6).
- Gouilleux, F. et al. (1994) EMBO J. 13, 4361-4369.
- Wakao, H. et al. (1994) EMBO J. 13, 2182-2191.
- Okutani, Y. et al. (2001) Oncogene 20, 6643-6650.
- Demoulin, J.B. et al. (1999) J. Biol. Chem. 274, 25855-25861.
- Dentelli, P. et al. (1999) J. Immunol. 163, 2151-2159.
- Meinke, A. et al. (1996) Mol. Cell. Biol. 16, 6937-6944.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.