Product Pathways - Screening Technologies
Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb #9607
|9607S||100 µl (10 western blots)||---||In Stock||---|
|9607||carrier free and custom formulation / quantity||email request|
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|W||1:1000||All Species Expected||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Phospho-ATM/ATR Substrate Motif (S*Q) (D23H2/D69H5) Rabbit mAb recognizes peptides and proteins containing sequences of phospho-Ser followed by Gln at the +1 position. The antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser containing motifs.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the S*Q motif sequence. The antibody is formulated from two rabbit monoclones in order to cover a broad range of reactivity.
Western blot analysis of extracts from HeLa cells, untreated (-) or UV-treated (+, 2 hr), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).
Immunoprecipitation of HeLa cells, untreated (-) or UV-treated (+, 2 hr) (lanes 3 and 4), using Phospho-ATM/ATR Substrate (S*Q) (D23H2/D69H5) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody (upper) and Chk1 (2G1D5) Mouse mAb #2360 (lower).
Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM/ATR is S*/T*Q. Hydrophobic amino acids at positions -3 and -1, and negatively charged amino acids at position +1 are positive determinants for substrate recognition by these kinases. Positively charged residues surrounding the S*/T*Q are negative determinants for substrate phosphorylation (3). The complex phenotype of AT cells suggests that it likely has additional substrates (3). To better understand the kinase and identify substrates for ATM and the related kinase ATR, CST has developed antibodies that recognize phosphorylated serine or threonine in the S*/T*Q motif.
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For Research Use Only. Not For Use In Diagnostic Procedures.
LI-COR® is a registered trademark of LI-COR, Inc.
Odyssey® is a registered trademark of LI-COR, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.