Revision 1

#96879

Store at -20C

Eosinophil cationic protein (F3G7N) & CO-0230-488 SignalStar® Oligo-Antibody Pair

1 Kit

(10 slides)

UniProt ID:
#P97426

Entrez-Gene Id:
#13586

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Product Includes Product # Volume Reactivity Isotype
Eosinophil cationic protein (F3G7N) Rabbit Monoclonal Antibody (SignalStar® Conjugate 0230)5323150 µlMRabbit IgG
Complementary Oligo (CO-0230-488)7568830 µl

Storage

SignalStar conjugates are supplied in PBS (pH 7.2), less than 0.1% sodium azide, 2 mM EDTA, 0.05% Triton X-100, 2 mg/mL BSA, and 50% glycerol. Complementary oligos are supplied in nuclease-free water. Store at -20°C. Do not aliquot the antibody. All components in this kit are stable for at least 12 months when stored at the recommended temperature.

Description

SignalStar multiplex immunohistochemistry (IHC) is an advanced technology for labeling multiple proteins simultaneously in tissue samples using specific primary antibodies and fluorescent detection reagents. This technology offers accuracy and reliability in visualizing and analyzing protein expression while maintaining spatial context and tissue architecture.

SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.

Specificity/Sensitivity

Eosinophil cationic protein (F3G7N) Rabbit mAb (SignalStar® Conjugate 0230) recognizes endogenous levels of total Eosinophil cationic protein. By overexpression, this antibody cross-reacts with mouse Eosinophil cationic-type ribonuclease 3/Ear3 protein but not Eosinophil cationic protein 2/Ear2, Eosinophil-associated ribonuclease 10/Ear10, or Eosinophil cationic-type ribonuclease 5/Ear5 proteins. This antibody detects 50 and 70 kDa bands of unknown origin in some cell lines and tissues. Non-specific staining was observed in crypts of mouse stomach and small intestine by immunohistochemistry.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro30 of mouse Eosinophil cationic protein.

Background

Eosinophil cationic protein (ECP, RNASE3) is a member of the ribonuclease superfamily which has both cytotoxic and noncytotoxic properties (1-4). ECP is primarily produced and stored in the secondary granules of eosinophils, where it is involved in host defense against parasites, bacteria, and viruses (1-4). It has also been detected in the primary granules of circulating neutrophils, and can be synthesized by stimulated neutrophils (3,4). ECP is often used as a marker for eosinophilic disease and is measurable in samples such as serum, bronchoalveolar lavage, and nasal secretions (4). A large number of clinical studies are focused on the role of ECP in eosinophil-related disorders, particularly in asthma (5).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

SignalStar is a registered trademark of Cell Signaling Technology, Inc.

U.S. Patent No. 10,781,477, foreign equivalents, and child patents deriving therefrom.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using Eosinophil cationic protein (F3G7N) Rabbit mAb (488; cyan), Granzyme B (E5V2L) Rabbit mAb (594; magenta), ASC/TMS1 (D2W8U) Rabbit mAb (647; red), CD8α (D4W2Z) Rabbit mAb (750; white), F4/80 (D2S9R) Rabbit mAb (647; green), CD206/MRC1 (E6T5J) Rabbit mAb (594; lilac), CD3ε (E4T1B) Rabbit mAb (750; yellow), and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 1: Eosinophil cationic protein (F3G7N) & CO-0230-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using Eosinophil cationic protein (F3G7N) Rabbit mAb (647; red) and DAPI #4083 (blue). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 2: Eosinophil cationic protein (F3G7N) & CO-0230-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded LL/2 syngeneic tumor using Eosinophil cationic protein (F3G7N) Rabbit mAb (left, 750; cyan) and DAPI #4083 (left, blue) compared to chromogenic immunohistochemical analysis of a serial section of paraffin-embedded LL/2 syngeneic tumor using Eosinophil cationic protein (F3G7N) Rabbit mAb #99106 (right). Staining was performed on the BOND RX autostainer by Leica Biosystems.
Immunohistochemistry Image 3: Eosinophil cationic protein (F3G7N) & CO-0230-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
SignalStar® oligo-based multiplex immunohistochemical analysis of paraffin-embedded mouse lung using Eosinophil cationic protein (F3G7N) Rabbit mAb (594; yellow) and DAPI #4083 (blue).
Immunohistochemistry Image 1: Eosinophil cationic protein (F3G7N) & CO-0230-488 SignalStar<sup>®</sup> Oligo-Antibody Pair
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.