Product Pathways - Metabolism
ACAD9 Antibody #9796
|9796S||100 µl (10 western blots)||---||In Stock||---|
|9796||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||69||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
ACAD9 Antibody recognizes endogenous levels of total ACAD9 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val512 of human ACAD9 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing DDK-tagged full-length human ACAD9 (hACAD9-DDK; +), using ACAD9 Antibody (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
The mitochondrial flavoenzymes acyl-CoA dehydrogenases (ACADs) catalyze the α,β dehydrogenation of acyl-CoA esters (1). One of these enzymes, ACAD9, is essential for assembly of oxidative phosphorylation complex I (2). Studies have shown that ACAD9 gene mutations cause Complex I deficiency (2,3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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