Product Pathways - Autophagy Signaling
NBR1 (D2E6) Rabbit mAb #9891
|9891S||100 µl (10 western blots)||---||In Stock||---|
|9891||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse||Endogenous||120||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
* Product-specific protocol.
Specificity / Sensitivity
NBR1 (D2E6) Rabbit mAb recognizes endogenous levels of total NBR1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys601 of human NBR1 protein.
Western blot analysis of extracts from various cell lines using NBR1 (D2E6) Rabbit mAb.
Flow cytometric analysis of MCF7 cells using NBR1 (D2E6) Rabbit mAb (blue) compared to concentration matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with bafilomycin A (0.2 μM, 18 hr; right), using NBR1 (D2E6) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Next to BRCA1 gene 1 (NBR1) protein is known for its encoding gene proximity to the BRCA1 tumor suppressor gene (1,2). N-terminal Phox and Bem1p (PB1) domains of NBR1 mediate its interaction with muscle specific titin kinase (3,4) and scaffolding protein p62 (4). NBR1 plays a role in autophagy by facilitating the autophagosomal degradation of ubiquitinated proteins independently and also in concert with p62 (5,6).
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