Revision 5

#98947

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IP, IHC-P, IF-F

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
60-70

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q8BHC0

Entrez-Gene Id:
114332

Product Usage Information

Application Dilution
Western Blotting 1:1000
Immunoprecipitation 1:200
Immunohistochemistry (Paraffin) 1:200 - 1:800
Immunofluorescence (Frozen) 1:200 - 1:800

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

Specificity/Sensitivity

LYVE-1 (E5P8S) Rabbit Monoclonal Antibody recognizes endogenous levels of total LYVE-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of mouse LYVE-1 protein.

Background

Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), also known as extracellular link domain-containing protein 1 (XLKD1), is a type I integral membrane glycoprotein. LYVE-1 is a CD44 homolog found primarily on lymphatic endothelial cells, but has also been described on hepatic blood sinusoidal endothelia, embryonic blood vessels, Kupffer cells, the islets of Langerhans, cortical neurons, and renal epithelium (1-3). LYVE-1 acts as a receptor for hyaluronan, and plays a role in hyaluronan transport and turnover (4-7). LYVE-1 also mediates the docking and entry of dendritic cells to lymphatic vessels through selective adhesion to hyaluronan in the leukocyte surface glycocalyx (8). Several studies have demonstrated the utility of LYVE-1 as a marker for lymphoid tissues and lymphangiogenesis (9,10).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

W: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Western blot analysis of extracts from mouse lymph node tissue, mouse lung tissue, and C2C12 cells using LYVE-1 (E5P8S) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).
Western Blotting Image 1: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse LYVE-1 protein (mLYVE-1-Myc/DDK; +), using LYVE-1 (E5P8S) Rabbit mAb (upper), Myc-Tag (71D10) Rabbit mAb #2278 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western Blotting Image 2: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Immunoprecipitation of LYVE-1 protein from mouse lung tissue extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is LYVE-1 (E5P8S) Rabbit mAb. Western blot analysis was performed using LYVE-1 (E5P8S) Rabbit mAb. Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) #93702 was used as a secondary antibody.
Immunoprecipitation Image 1: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded mouse liver using LYVE-1 (E5P8S) Rabbit mAb.
Immunohistochemistry Image 1: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse prostate using LYVE-1 (E5P8S) Rabbit mAb.
Immunohistochemistry Image 2: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded Renca syngeneic tumor using LYVE-1 (E5P8S) Rabbit mAb.
Immunohistochemistry Image 3: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Immunohistochemical analysis of paraffin-embedded CT26 syngeneic tumor using LYVE-1 (E5P8S) Rabbit mAb.
Immunohistochemistry Image 4: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded mouse lung using LYVE-1 (E5P8S) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemistry Image 5: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Immunohistochemical analysis of paraffin-embedded 293T cell pellet, untransfected (left) or mouse Lyve1-transfected (right), using LYVE-1 (E5P8S) Rabbit mAb.
Immunohistochemistry Image 6: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 5

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with LYVE-1 (E5P8S) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #27394 (cyan pseudocolor), beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #23371 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 1: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with LYVE-1 (E5P8S) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #27394 (cyan pseudocolor), beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #23371 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Immunofluorescence Image 2: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Confocal immunofluorescent analysis of fixed frozen mouse pancreas labeled with LYVE-1 (E5P8S) Rabbit Monoclonal Antibody (green). Free secondary binding sites were then blocked with Rabbit (DA1E) Monoclonal Antibody IgG Isotype Control #3900 prior to co-labeling with CD31 (PECAM-1) (F2N3M) Rabbit Monoclonal Antibody (Alexa Fluor® 555 Conjugate) #27394 (red) and beta-Catenin (D10A8) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate) #23371 (blue).
Immunofluorescence Image 3: LYVE-1 (E5P8S) Rabbit Monoclonal Antibody
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.