Background
Vinblastine is a vinca alkaloid derived from the plant Catharanthus roseus. Like other vinca alkaloids, it acts as a mitotic inhibitor blocking microtubule assembly in vitro (1,2). Investigators have demonstrated that vinblastine binds to and inhibits the addition of tubulin dimers to the assembly end of steady-state microtubules in a dose-dependent manner (Ki = ~178 nM) (1). This disruption of mitotic spindle causes mitotic arrest, inhibits cell proliferation, and induces apoptosis (1-3). Studies have suggested that high concentrations of vinblastine depolymerize microtubules, but this is not required for inhibition of cell proliferation (2). Treatment of cells with vinblastine has been shown to activate the SAPK/JNK pathway, leading to expression and phosphorylation of c-Jun (3,4) and phosphorylation of the anti-apoptotic proteins Bcl-2 and Bcl-xL (5,6).
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- Kolomeichuk, S.N. et al. (2008) FEBS J 275, 1889-99.
- Duan, L. et al. (2007) Biochem Pharmacol 73, 481-90.
- Fan, M. et al. (2000) J Biol Chem 275, 29980-5.
- Poruchynsky, M.S. et al. (1998) Cancer Res 58, 3331-8.
Molecular Formula
C46H58N4O9•H2SO4
Molecular Weight
909.05 g/mol
Purity
>98%
CAS
143-67-9
Solubility
Soluble in DMSO at 50mg/ml and H2O at 10mg/ml.
Storage
Directions for Use
Vinblastine is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 5 mg in 550 μl DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 10-1000 nM for 12-48 hr.
Background References
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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