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Product Usage Information

TPA is supplied as a 200 μM stock in DMSO. For standard 200 nM treatment, dilute 1000X.

Storage: Store lyophilized or in solution at -20°C. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.

Product Description

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20ºC, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling.

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).

1.  Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31.

2.  Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97.

3.  Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6.

4.  Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61.

5.  Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31.

6.  Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5.

Data Sheets & Documentation

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

TPA (Phorbol-12-Myristate-13-Acetate)