Fresh savings just popped up! 25% off 3 or more products. | Start Saving >>
TPA (Phorbol-12-Myristate-13-Acetate)

TPA (Phorbol-12-Myristate-13-Acetate) #9905

This product is discontinued

We recommend the following alternatives

TPA is supplied as a 200 μM stock in DMSO. For standard 200 nM treatment, dilute 1000X.


Store lyophilized or in solution at -20°C. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency.

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is soluble in DMSO and ethanol. DMSO solutions can be stored at -20ºC, in the absence of light, for at least six months. A 200 nM TPA treatment is standard to induce signaling.

TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6).

  1. Rebois, R.V. and Patel, J. (1985) J Biol Chem 260, 8026-31.
  2. Blumberg, P.M. (1980) Crit Rev Toxicol 8, 153-97.
  3. Fürstenberger, G. et al. (1981) Proc Natl Acad Sci U S A 78, 7722-6.
  4. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5357-61.
  5. Lee, H.W. et al. (2002) J Biol Chem 277, 32624-31.
  6. Han, Z.T. et al. (1998) Proc Natl Acad Sci U S A 95, 5362-5.
For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

News from the Bench

Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.