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78679
BAY 11-7082
Activators & Inhibitors
Chemical Modulators

BAY 11-7082 #78679

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BAY 11-7082: Image 1

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 5 min; +) and Calyculin A #9902 (50 nM, 5 min; +) with or without pretreating the cells with BAY 11-7082 (5 μM, 1 hr; +), using Phospho-NF-κB p65 (Ser468) Antibody #3039 (upper), NF-κB p65 (D14E12) XP® Rabbit mAb #8242 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

BAY 11-7082: Image 2

Chemical structure of BAY 11-7082.

Product Usage Information

BAY 11-7082 is supplied as a lyophilized powder. For a 20 mM stock, reconstitute 5 mg of powder in 1.2 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.

Storage

Store lyophilized at room temperature, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 207.3 g/mol
Purity

>98%

Molecular Formula

C10H9NO2S

CAS 19542-67-7
Solubility Soluble in DMSO at 25 mg/ml or ethanol at 15 mg/ml.

Background

BAY 11-7082 is a selective and irreversible inhibitor of cytokine-induced IκBα phosphorylation. Treatment with TNF-α leads to the activation of IκBα resulting in nuclear translocation of NF-κB where it regulates gene expression. Inhibition of IκBα phosphorylation leads to the inactivation of NF-κB (1). The effect that BAY 11-7082 has on proinflammatory cytokine production as it relates to the NF-κB/IκB kinase pathways makes it useful when studying inflammation (2). This compound inhibits IFN-α production and nuclear translocation of interferon regulatory factor 7 (IRF7) in human plasmacytoid dendritic cells, both important factors when studying immunotherapeutic strategies as they relate to autoimmune disorders (3). BAY 11-7082 inhibits TNF-α-induced surface expression of ICAM-1, VCAM-1, and E-Selectin in human endothelial cells with an IC50 value between 5–10 μM (1).

  1. Pierce, J.W. et al. (1997) J Biol Chem 272, 21096-103.
  2. Lappas, M. et al. (2005) Endocrinology 146, 1491-7.
  3. Miyamoto, R. et al. (2010) Arthritis Res Ther 12, R87.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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To Purchase # 78679S
Product # Size Price
78679S
5 mg $ 59