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Activators & Inhibitors
Chemical Modulators

Beta-d-N4-Hydroxycytidine #81178

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other Image 1 - Beta-d-N4-Hydroxycytidine

Chemical structure of Beta-d-N4-Hydroxycytidine.

Product Usage Information

Beta-d-N4-Hydroxycytidine is supplied as a lyophilized powder. For a 15 mM stock, reconstitute 5 mg of powder in 1.28 ml of DMSO. Working concentrations and length of treatment can vary depending on the desired effect.


Store lyophilized at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, store at -20ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 259.2 g/mol


Molecular Formula


CAS 3258-02-4
Solubility Soluble in DMSO at 25 mg/ml or water at 15 mg/ml with slight warming.


Beta-d-N4-Hydroxycytidine, also known as EIDD-1931, is a nucleic acid base analog and a specific AT to GC mutagen formed by the treatment of nucleoside cytidine with hydroxylamine (1). Beta-d-N4-Hydroxycytidine acts as a competitive substrate for viral encoded RNA polymerase and has demonstrated broad in vitro antiviral activity, including Chikungunya virus, Ebola, and Hepatitis C (2-4). Beta-d-N4-Hydroxycytidine demonstrated an IC50 value of 0.1 µM and an IC90 value of 6 µM in SARS-CoV infected Vero cells (5,6).

  1. Janion, C. and Glickman, B.W. (1980) Mutat Res 72, 43-7.
  2. Ehteshami, M. et al. (2017) Antimicrob Agents Chemother 61, e02395-16. doi: 10.1128/AAC.02395-16.
  3. Reynard, O. et al. (2015) Viruses 7, 6233-40.
  4. Stuyver, L.J. et al. (2003) Antimicrob Agents Chemother 47, 244-54.
  5. Barnard, D.L. et al. (2006) Antivir Chem Chemother 17, 275-84.
  6. Barnard, D.L. et al. (2004) Antivir Chem Chemother 15, 15-22.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 81178S
Product # Size Price
5 mg $ 95