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13637
Camptothecin
Activators & Inhibitors
Chemical Modulators

Camptothecin #13637

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Camptothecin: Image 1

Chemical structure of camptothecin.

Camptothecin: Image 2

Western blot analysis of extracts from MCF7 cells, untreated (-) or treated with Camptothecin (6 hr) at the indicated concentrations, using Phospho-p53 (Ser15) Antibody #9284 (upper), p53 (1C12) Mouse mAb #2524 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Product Usage Information

Camptothecin is supplied as a lyophilized powder. For a 10 mM stock, reconstitute the 10 mg in 2.87 ml DMSO. Working concentrations and length of treatment can vary depending on the desired effect, but it is typically used at 1-10 µM for 1-24 hr.

Storage

Store lyophilized or in solution at -20ºC, desiccated. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Product Description

Molecular Weight 348.4 g/mol
Purity

>98%

Molecular Formula

C20H16N2O4

CAS 7689-03-04
Solubility Soluble in DMSO at 10mg/ml.

Background

Camptothecin is a cytotoxic plant alkaloid originally isolated from C. acuminate that inhibits DNA and RNA synthesis in mammalian cells and is an effective anti-tumor agent (1). Research studies indicate that camptothecin inhibits topoisomerase I with an IC50 of 679 nM (2). Camptothecin binds and stabilizes topoisomerase I–DNA cleavage complexes, which leads to DNA strand breaks (1,3,4). The resultant DNA damage can induce cell cycle arrest in many cancer cell lines (5,6). Inactivation of the tumor suppressor protein p53 can increase the cytotoxicity of camptothecin (6).

  1. Hsiang, Y.H. et al. (1985) J Biol Chem 260, 14873-8.
  2. Luzzio, M.J. et al. (1995) J Med Chem 38, 395-401.
  3. Marchand, C. et al. (2006) Mol Cancer Ther 5, 287-95.
  4. Jaxel, C. et al. (1991) J Biol Chem 266, 20418-23.
  5. Jaks, V. et al. (2001) Oncogene 20, 1212-9.
  6. Gupta, M. et al. (1997) Clin Cancer Res 3, 1653-60.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
To Purchase # 13637S
Product # Size Price
13637S
10 mg $ 42