News from the Bench
Discover what’s going on at CST, receive our latest application notes and tips, read our science features, and learn about our products.
the purchase of 3 or more products
To Purchase # 9973S
Find answers on our FAQs page.
- Additional protein information
- Analytical tools
Cyclosporin A #9973
Gallery: Cyclosporin A #9973
Cyclosporin A is supplied as a 100 mg powder. Store at -20ºC. Cyclosporin A is soluble in ethanol at 200 mg/ml (also DMSO at 100 mg/ml) and stock solutions should be stored at -20ºC. Working concentrations and length of treatment can vary depending on the desired effect but it is typically used at around 100 nM.Storage: Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.
The calcium dependent protein phosphatase calcineurin is responsible for the de-phosphorylation of the transcriptional regulator nuclear factor of activated T cells (NFAT) and is essential for NFAT’s nuclear translocation and activation (1,2). Calcineurin is a target of two common immunosuppressants, cyclosporin A (CsA) (3) and FK-506 (also know as tacrolimus and fugimycin) (4), both of which can inhibit antigen and mitogen triggered T cell activation. These drugs interact with the immunophilins cyclophilin and FKBP-12, respectively, and the immunophilin-drug complex binds to calcineurin to inhibit substrate binding (5). FK-506 can be up to 100-fold more potent than CsA in various models (6-8).
For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.