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Forskolin #3828

Western Blotting

Western blot analysis of extracts from SH-SY5Y cells, forskolin-treated (30uM for 15 min.) or untreated, using Phospho-Creb (Ser133) Rabbit mAb #9198 (upper) or CREB Rabbit mAb #9197 (middle).

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For 30 mM stock, reconstitute 10 mg in 812 μl DMSO.


Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles.

Molecular Weight:

410.5 g/mol



Molecular Formula:


Forskolin, a naturally occurring diterpene from the Indian plant, Coleus forskohlii, activates adenylate cyclase, and thus increases the intracellular cAMP concentration (1). The second messenger cAMP activates cAMP-dependent protein kinase (PKA or cAPK) and controls many cellular mechanisms such as gene transcription, ion transport and protein phosphorylation (2).

  1. Laurenza, A. et al. (1989) Trends Pharmacol Sci 10, 442-7.
  2. Montminy, M. (1997) Annu Rev Biochem 66, 807-22.
For Research Use Only. Not For Use In Diagnostic Procedures.

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